Hydrodynamic Fractionation of Macromolecules. I. A Simple Theory
作者:
F.H. Verhoff,
N.D. Sylvester,
期刊:
Journal of Macromolecular Science: Part A - Chemistry
(Taylor Available online 1970)
卷期:
Volume 4,
issue 4
页码: 979-1001
ISSN:0022-233X
年代: 1970
DOI:10.1080/00222337008060992
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new mechanism for separation in gel permeation chromatography (CPC) or gel filtration has been developed based upon the postulate that flow occurs through the gel phase. On the basis of the hydrodynamic nature of the separation mechanism, the new nameHydrodynamic Fractionationis proposed. A theory has been formulated for the case of equal pore size in the gel phase and equal sized spaces between the beads. This theory predicts the elution volume vs molecular weight curve. The flow-rate dependence of the separation peaks is investigated.
点击下载:
PDF (823KB)
返 回