Glucocorticoid Receptor Concentration Modulates Glucocorticoid-Regulated Gene Expression in Rat Pancreatic AR42J Cells
作者:
Astrid Kaiser,
Ute Stier,
Ernst-Otto Riecken,
Stefan Rosewicz,
期刊:
Digestion
(Karger Available online 1996)
卷期:
Volume 57,
issue 3
页码: 149-160
ISSN:0012-2823
年代: 1996
DOI:10.1159/000201329
出版商: S. Karger AG
关键词: Glucocorticoid receptor;Amylase;Bombesin receptor;Pancreas;Acinar cell
数据来源: Karger
摘要:
In this study we investigated the effects of altered intracellular glucocorticoid receptor (GR) concentrations on glucocorticoid-regulated gene expression in the rat pancreatic acinar cell line AR42J. Incubation of AR42J cells with dexa-methasone results in a time-dependent transcriptional stimulation of amylase gene expression (about 5-fold) and a transcriptional inhibition of bombesin receptor (BR) gene expression. Decreasing the intracellular GR concentration to 50% by preincubation with RU 38486 results in a significant attenuation of dexamethasone-regulated amylase and BR gene expression. In contrast, increasing the intracellular GR concentration 2-fold by preincubation with the phosphodiesterase inhibitor IBMX significantly enhances the glucocorticoid inhibition of BR gene expression whereas amylase mRNA concentrations remain unaltered. These data demonstrate that intracellular GR concentrations determine the sensitivity of glucocorticoid-regulated gene expression in rat pancreatic AR42J cells.
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