The Effect of Castration on the Synthesis and Secretion of Proteins in 7,12-Dimethylbenz(a)anthracene-Induced Rat Mammary Tumors
作者:
GeierA.,
HemiR.,
BeeryR.,
LunenfeldB.,
期刊:
Cancer Investigation
(Taylor Available online 1992)
卷期:
Volume 10,
issue 3
页码: 209-213
ISSN:0735-7907
年代: 1992
DOI:10.3109/07357909209032762
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The effect of castration on the incorporation of p5S]methionine into secreted proteins in 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary tumors, was investigated. Biopsy specimens were obtained from 19 tumors, 0, 24, 48, 72, and 96 h after castration. In 14 tumors, castration induced an increase in the incorporation (mean of 5-fold), reaching the maximal level after 24 h (3 tumors), 48 h (7 tumors), 72 h (3 tumors), and after 96 h (1 tumor). In three tumors castration did not alter the incorporation rate, while in two tumors incorporation declined immediately after castration. One-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the labeled secreted protein showed that castration did not decrease or increase significantly the incorporation of [35S]methionine into any of the major labeled proteins. Conclusion: tumor regression following hormonal deprivation is apparently preceded by an increased synthesis of secreted proteins. However, no qualitative differences in any major labeled proteins could be observed.
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