McArdle's&Hers' diseases: Glycogen phosphorylase transcriptional expression in human tissues
作者:
GorinFrederic A.,
MullinaxRebecca L.,
IgnacioPamela C.,
NeveRachael L.,
KurnitDavid M.,
期刊:
Journal of Neurogenetics
(Taylor Available online 1987)
卷期:
Volume 4,
issue 4
页码: 293-308
ISSN:0167-7063
年代: 1987
DOI:10.3109/01677068709167190
出版商: Taylor&Francis
关键词: McArdle's disease;Hers' disease;Glycogen phosphorylase isozyme;Transcriptional expression;Human and primate tissues
数据来源: Taylor
摘要:
We have cloned the cDNA encoding human liver glycogen phosphorylase (glycogenosis type VI) from a fetal brain cDNA library. Liver (L) and muscle (M) phosphorylase cDNA probes were used to determine the relative abundance of mRNA encoding the L- and M-isozymes of phosphorylase in human fetal and adult tissues. The transcript encoding the M-isozyme is 3.4 kb; the L-isozyme transcript is 3.3 kb. Transcriptional expression of the L-isozyme in human and primate tissues was found to differ from the isozyme's reported tissue specificity in non-primate mammals. Furthermore, using degenerate oligonucleotide probes to two different coding regions of M-phosphorylase, a novel 4.1-kb transcript was demonstrated to be present in human fetal and adult brain.
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