Collagen Production by Rat Hepatocytes and Sinusoidal Cells in Primary Monolayer Culture
作者:
Scheffer C. G. Tseng,
Philip C. Lee,
Peter F. Ells,
D. Montgomery Bissell,
Edward A. Smuckler,
Robert Stern,
期刊:
Hepatology
(WILEY Available online 1982)
卷期:
Volume 2,
issue 1
页码: 13-18
ISSN:0270-9139
年代: 1982
DOI:10.1002/hep.1840020104
出版商: W.B. Saunders
数据来源: WILEY
摘要:
AbstractThe cellular sources of collagen in normal rat liver have been examined. Hepatocytes and nonparenchymal (sinusoidal) cells were isolated and established in primary monolayer culture. These cells were incubated with radiolabeled proline in the presence of L‐ascorbate and β‐amino‐propionitrile. Nondialyzable material was prepared from the cell layer and the medium from each type of culture. The level of collagen accumulation was determined by measuring labeled hydroxyproline and sensitivity to purified bacterial collagenase. In hepatocytes, collagen represented 0.2% of both secreted and cell‐associated labeled protein. In sinusoidal cells, collagen was 3.2% of secreted and 1.1% of cell‐associated proteins. The total secreted labeled collagen, expressed per microgram of DNA, was similar in hepatocytes and sinusoidal cells. However cell‐associated collagen in hepatocyte culture was approximately 10‐fold that present in sinusoidal cells. These findings indicate that, while collagen formation is a relatively important function of sinusoidal cells, in normal liver the contribution of hepatocytes to total hepatic collagen accumulation may
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