AMINO ACID ANALYSIS OF PEPTIDES USING HPLC WITH EVAPORATIVE LIGHT SCATTERING DETECTION
作者:
JeffreyA. Peterson,
LeslieJ. Lorenz,
DonaldS. Risley,
BeverlyJ. Sandmann,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 7
页码: 1009-1025
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101714
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A new procedure for the amino acid analysis of peptides has been devised utilizing high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD). This procedure eliminates the need for complex derivatization schemes inherent of previous amino acid analysis procedures since the ELSD detects the amino acids directly. This quantitative method detects and separates 18 of the common amino acids in a one hour run time using cation exchange chromatography coupled with ELSD. The procedure was tested by analyzing the hydrolysate of human parathyroid hormone 1–34 (PTH), a synthetic polypeptide. A standard digestion consisting of 24 hour hydrolysis at 110°C in 6 N hydrochloric acid with 3% phenol was used. Validation data reveal this is an accurate and precise procedure for the amino acid analysis of peptides.
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