Electron microscopy of mouse preimplantation embryos and isolated blastomeres immobilized on coverslips
作者:
Lynn M. Wiley,
Kathy K. Takaki,
Maya Yamagata,
期刊:
Gamete Research
(WILEY Available online 1985)
卷期:
Volume 11,
issue 1
页码: 51-58
ISSN:0148-7280
年代: 1985
DOI:10.1002/mrd.1120110106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: preimplantation embryo;electron microscopy;blastomeres;cell adhesion
数据来源: WILEY
摘要:
AbstractIn this report we describe a new method of immobilizing intact mouse preimplantation embryos and their dissociated blastomeres on a flat substrate so that they may be processed en masse for electron microscopy. The embryos can be arranged so that they will be coplanar and grouped together closely enough to be included within the same plane of section. The substrate consists of Thermonex plastic coverslips that have been coated with a layer of fibroblasts or with fibronectin. The plant lectin phytohemaggiutinin (PHA) is used as a ligand to adhere the embryos onto the coverslips. This method is compatible with other ligands and with a variety of immunocytochemical procedures and can be used with live or fixed embryos.
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