Immunohistochemical Detection of Estrogen Receptors in Paraffin Sections of Human Thyroid Tissues
作者:
Yoshio Hiasa,
Hiroto Nishioka,
Yoshiteru Kitahori,
Katsunari Yane,
Shingo Nakaoka,
Masato Ohshima,
Noboru Konishi,
Kiyoharu Nishii,
Motokazu Kitamura,
Masahito Sugimura,
期刊:
Oncology
(Karger Available online 1991)
卷期:
Volume 48,
issue 5
页码: 421-424
ISSN:0030-2414
年代: 1991
DOI:10.1159/000226972
出版商: S. Karger AG
关键词: Estrogen receptor;Human thyroid tumor;Immunohistochemistry;Paraffin section
数据来源: Karger
摘要:
The optiomal demonstration of estrogen receptor binding in thyroid tissues was made under conditions of 10% protease in 50 mM Tris-HCl buffer (pH 7.6) for 10 min as the pretreatment digestion step, incubation of primary antibody (ER-ICA monoclonal kit; Abbott Laboratories) at 37 °C for 2 h and incubation of secondary antibody (ABC kit; Vector) at 37 °C for 40 min. Thyroid tissues used for assessing the reaction were 17 cases of goiter, 25 adenoma cases, 27 cases of papillary carcinoma, 14 cases of follicular carcinoma and 10 latent cancer cases. Incidences of positive estrogen receptor reaction were 22% (11/51) for all thyroid cancers, 20% (5/25) for the thyroid adenomas and 59% (10/17) for goiters. 15% (4/27) of papillary carcinomas, 21% (3/14) of follicular carcinomas and 40% (4/10) of latent cancers proved positive, the estrogen receptor reaction being limited to the nuclei of thyroid follicular/papillary type cell
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