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Rapid Three-Dimensional Reconstruction at the Light Microscopic Level and a Technique for Re-Embedding the Same Semithin Sections for Electron Microscopic Examination

 

作者: PignotI.,   BressacC.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1992）
卷期: Volume 67, issue 1  

页码: 55-57

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110007

 

出版商: Taylor&Francis

 

关键词: Drosophila;electron microscopy;light microscopy;spermatozoa;three-dimensional reconstruction

 

数据来源: Taylor

 

摘要:

Rapid three-dimensional reconstruction of serial sections at the light microscopic and ultrastructural levels was accomplished using a two-step technique. Fixed specimens were embedded in Epon and 1μm sections were cut and placed on glass slides. One of every four sections was drawn onto transparency film for rapid three-dimensional reconstruction. The semi-thin sections were re-embedded in Epon and sectioned at 90 nm for examination in the electron microscopy.

 

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