Shoots were induced at a very high frequency (85.6%) from dormant axillary buds ofPopulus tremulaL. Exogenous phytohormones were not necessary for shoot induction but an addition of BAP (benzylaminopurine) in media increased somewhat the number of regenerating buds. Effects on shoot regeneration of the vitamin content of the media as well as of the season of bud collection were confirmed. Micropropagating coefficient values in regenerated aspen shoots varied from 6.6 to 23.8 depending on clonal variations and cultural conditions. 96.0% of cultured shoots differentiated roots within 10 days and the rooted plantlets were easily transplanted to the greenhouse.