Purpose. To investigate the metabolic profile of the rabbit lens using high resolution1H NMR spectroscopy including two-dimensional shift correlated (COSY) technique.Methods. Perchloric acid extracts of the rabbit lens were analysed with a Bruker AM-500 spectrometer and the metabolites were assigned in the spectra. Some of these were also quantified.Results. More than 20 metabolites were detected in the perchloric acid extract of a single lens, including amino acids, nucleotides and other related compounds. Of particular importance is the ability to detect and identify glutathione, myo-inositol, scyllo-inositol and taurine.Conclusions. The study demonstrated the potential of1H NMR spectroscopy for monitoring the metabolic profile of the lens in normal and pathologic conditions.