High-Performance Liquid Chromatographic Determination of SAZ-VII-23, A Novel Antiarrhythmic Agent, in Dog Plasma and Urine
作者:
C.L. Chen,
J.D. Roder,
S. Sangiah,
H. Chen,
K.D. Berlin,
G.L. Garrison,
B.J. Scherlag,
R. Lazzara,
E. Patterson,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 6
页码: 1125-1135
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308019892
出版商: Taylor & Francis Group
关键词: HPLC;antiarrhythmic;pharmacokinetics;nonane
数据来源: Taylor
摘要:
A HPLC method has been developed to determine the concentrations of SAZ-VII-23 (3-benzoyl-7-isopropyl-3,7-diazabicyclo[3.3.1]nonane HClO4), a novel antiarrhythmic agent, in dog plasma and urine. Plasma treated with acetonitrile and alkalinized urine were extracted with chloroform- propanol (9:1). An aliquot was injected on to HPLC system using a C6reversed-phase column and acetonitrile-methanol-37.5 mM phosphate buffer, pH 6.8 (28.5:28.5:43 v/v) containing 4.0 mM triethylamine as mobile phase. Detection wavelength was 255 nm. The linear range were 0.04–8 μg/ml, and the lower limit of quantitation was 0.04 μg/ml in plasma and urine, respectively. The method was applied to determine plasma and urine concentrations and preliminary pharmacokinetic profiles of SAZ-VII-23 in a dog.
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