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The culturing of dissociated newt forelimb regenerate cells

 

作者: Joseph A. Jabaily,   Patricia Blue,   Marcus Singer,  

 

期刊: Journal of Experimental Zoology  （WILEY Available online 1982）
卷期: Volume 219, issue 1  

页码: 67-73

 

ISSN:0022-104X

 

年代: 1982

 

DOI:10.1002/jez.1402190108

 

出版商: Wiley Subscription Services, Inc., A Wiley Company

 

数据来源: WILEY

 

摘要:

AbstractA procedure is described for the mechanical dissociation and culturing of early stage newt regenerate blastema cells. The specified growth medium is adjusted to suitable osmolarity and consists of Leibovitz L‐15, water, fetal calf serum, and insulin. This provides a suitable environment in which growth, proliferation, and some differentiation occur with time as evidenced by phase contrast photographs of living culture

 

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