An elctrophoretic analysis of 26 streptomyces strains by 10 enzymes has been performed. Seven enzyme loci assayed have been polymorphic. For each of the enzymes hexokinase and malatdehydrogenase one allele has been registered. The electrophoretic mobility for HEX have been the same for all the studied strains. The investigated streptomyces strains divide into two cluster groups—A and B according to their Rf values. The type strains of the well defined according to Williams four species S. albidoflavus, S. albus, S. fradiae and S. griseus, divide into different clusters. From the species with not definite taxonomic status, S. floridae and S. acrimicini, together with S. griseus (type strain), allocate in one cluster group, as well as the numeric-taxonomic investigation of Kampfer. S. gipsonii, S. rangoon, S. somaliensis also belong to group B. The rest six studied strains with not definite taxonomic status fall into group A, where the mostly heterogeneous subcluster is A2-l, including the type strain of S. albus.