Evidence has accumulated suggesting acetylcholine may he involved in initiation of the heart heat. Acetylcholine and electrolyte metabolism in the various chambers of the heart have been studied. The area of highest intrinsic rhythm (sinus) contained greater amounts of acetylcholine equivalents, true cholinesterase, choline acetylase and sodium; all of these decreasing in amount in areas of lower intrinsie rhythm. It is concluded that intrinsic rhythm may he directly correlated with overall acetylcholine metabolism and sodium content and inversely related to overall energy metabolism and potassium concentration.