Deoxythymidine Kinase from Rabbit Kidney Cells Infected with Herpes Simplex Virus Types 1 and 2
作者:
Takeo Ogino,
Ross Shiman,
Fred Rapp,
期刊:
Intervirology
(Karger Available online 1973)
卷期:
Volume 1,
issue 2
页码: 80-95
ISSN:0300-5526
年代: 1973
DOI:10.1159/000148835
出版商: S. Karger AG
关键词: Herpes simplex virus;Thymidine kinase;Rabbit kidney cells
数据来源: Karger
摘要:
Deoxythymidine kinase (TdR kinase) from rabbit kidney cells infected in vitro with herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2) was further investigated. Enzyme activity induced by HSV-2 was more heat-labile and more sensitive to inhibition by deoxythymidine nucleotides than HSV-1 induced enzyme activity in both crude extracts and partially purified preparations. The thermostability of TdR kinase activity from cells coinfected with HSV-1 and HSV-2 was intermediate between enzymes induced separately. The difference in thermostability between the two enzyme activities was also observed in extracts from 1-β-D-arabinofuranosylcytosine (ara-C) treated infected cells. HSV-1 and HSV-2 induced enzymes also differed in their response to substrate concentration. Gel filtration with Sephadex G-100 revealed the presence of monomer, dimer and aggregated forms of HSV-1 induced enzyme. The molecular weights of the monomer and dimer were estimated to be 58,000 and 97,000, respectively. Such multiple forms were not observed with HSV-2; the molecular weight of the HSV-2 induced enzyme was estimated to be 45,000 to 60,000, depending upon the eluting buffers. HSV-1 induced TdR kinase activity showed a different electrophoretic activity pattern in polyacrylamide gel electrophoresis than did the enzyme induced by HSV-2
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