Changes in contractile protein gene expression with ageing and with captopril‐induced regression of hypertrophy in the spontaneously hypertensive rats
作者:
Geoff Dalton,
John Jones,
Allan Levi,
Andrew Levy,
期刊:
Journal of Hypertension
(OVID Available online 2000)
卷期:
Volume 18,
issue 9
页码: 1297-1306
ISSN:0263-6352
年代: 2000
出版商: OVID
关键词: spontaneously hypertensive rat;ageing;hypertrophy;regression;contractile protein;gene expression;captopril;angiotensin converting enzyme inhibitor
数据来源: OVID
摘要:
BackgroundLeft ventricular hypertrophy (LVH) is present in young spontaneously hypertensive rats (SHR) compared with normotensive Wistar–Kyoto (WKY) rats and treatment of SHR with captopril leads to regression of LVH. Hypertrophy produces changes in gene expression for myofibrillar proteins with increased ratios of skeletal to cardiac actin and β to α-myosin heavy chain (MHC).ObjectivesThe objective of this study was to follow changes in transcript prevalence for these four proteins during ageing and with captopril treatment in SHR and WKY rats.MethodsUntreated SHR and WKY rats were studied at 100, 156, 350 and 450 days. Groups at 100 and 350 days were divided into a treatment group (given captopril) and untreated controls. Transcripts were measured usingin situhybridization.ResultsBoth cardiac and skeletal actin were increased in untreated SHR compared to WKY rats (P< 0.01 andP< 0.05, respectively). α-MHC was increased (P< 0.01) whilst β-MHC was normal in 100-day-old SHR (an age when LVH was present) compared with WKY rats. With ageing, α-MHC declined and β-MHC increased giving the increased ratio of β to α-MHC transcripts reported by other investigators. Treatment of SHR led to a significant decline in skeletal actin transcripts (P< 0.01) and reversed the rise in β-MHC expression that occurred with ageing (P< 0.01).ConclusionsLVH in SHR is associated with increased skeletal and cardiac actin transcripts. Despite unequivocal LVH in SHR at 100 days of age, α rather than β-MHC transcripts were increased. Only with ageing did the classically reported increased ratio of β to α-MHC transcripts become apparent. Captopril treatment reduced skeletal actin transcripts and reversed the increase in β-MHC that occurred with ageing.
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