TOPICAL INTERLEUKIN 1 RECEPTOR ANTAGONIST PROMOTES CORNEAL TRANSPLANT SURVIVAL1
作者:
Dana2 M.,
Yamada2 Jun,
Streilein3 J.,
期刊:
Transplantation
(OVID Available online 1997)
卷期:
Volume 63,
issue 10
页码: 1501-1507
ISSN:0041-1337
年代: 1997
出版商: OVID
数据来源: OVID
摘要:
Background.Interleukin (IL)-1 is a potent proinflammatory cytokine that plays a critical role in initiating and maintaining immunogenic inflammation. We performed a series of experiments to determine whether the topical application of IL-1 receptor antagonist (IL-1ra) can prolong corneal transplant survival in the murine model of orthotopic allotransplantation.Methods.For all experiments, C57BL/6 corneas were transplanted into BALB/c (major histocompatibility and minor histocompatibility-disparate) eyes. “High-risk” transplants were transplants that had been sutured into BALB/c recipient beds with corneal neovascularization induced by placement of three interrupted sutures in the host cornea 2 weeks earlier. Both risk groups were divided in a masked fashion into treatment subgroups that received either 20 mg/ml of IL-1ra mixed in 0.2% sodium hyaluronate vehicle (n=28) or placebo alone (n=25). All transplants were evaluated for 8 weeks after surgery for signs of rejection. At the end of follow-up, corneal specimens were processed for enumeration of Langerhans cells and histopathological evaluation.Results.Survival rates of both normal-risk and high-risk transplants increased significantly among the IL-1ra-treated animals compared with untreated controls by both stratified Mantel-Haenszel (P=0.02) and Kaplan-Meier survival (P=0.03) analyses. Furthermore, both normal- and high-risk IL-1ra-treated grafts had significantly less inflammation and Langerhans cells infiltration compared with untreated controls.Conclusions.Topical treatment with IL-1ra has a significantly positive effect in promoting corneal allograft survival.
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