A study was conducted to examine the genetic variability for tolerance to aluminum toxicity in several Kenyan cultivars. The F2 seedlings from eleven crosses between tolerant and intolerant cultivars exhibited a wide range of segregation ratios and indicated that aluminum tolerance in this group of parents was conditioned by two or more genes in each cross. Four of the individual crosses fitted a two‐gene model, and none fitted a single gene model. Expression of the tolerance genes in the F2 progeny was dependent on the genetic background. In three out of four crosses between tolerant parents, a high frequency of susceptible segregates was obtained. The segregation ratio tests performed on the F2 seedling populations suggested that the tolerant parents Romany, PF7748, K. Kongoni and K. Tembo had different tolerance genes. The results from crosses involving K. Fahari, K. Swara and Siete Cerros (all susceptible) indicated that aluminum susceptibility was controlled by recessive genes. The F2 progeny from the crosses K. Tembo x Siete Cerros, K. Swara x Romany, K. Kongoni x PF7748, and K. Fahari x PF7748 fitted a 9 tolerant: 7 susceptible ratio, with dominant genes controlling tolerance. F2 test results indicated that new sources of aluminum tolerance could be incorporated into Kenyan wheat cultivars. Results also suggest that the cultivar Romany is almost equal in tolerance to the best aluminum tolerant spring wheat cultivars previously reported in the literature, and that it is equally effective as a donor parent for aluminum tolerance.