Subcellular Localization of Hypothalamic Luteinizing Hormone
作者:
Nicholas Emanuele,
Sergei Lipov,
James Anderson,
Ellen Andersen,
Lidia Kirsteins,
Nancy Nishimura,
Ann M. Lawrence,
期刊:
Neuroendocrinology
(Karger Available online 1985)
卷期:
Volume 40,
issue 4
页码: 339-344
ISSN:0028-3835
年代: 1985
DOI:10.1159/000124096
出版商: S. Karger AG
关键词: Luteinizing hormone;Hypothalamus;Brain;Synaptosome
数据来源: Karger
摘要:
Ultracentrifugation and sucrose gradient separation techniques have been applied to study of the subcellular distribution of hypothalamic luteinizing hormone (LH). Subcellular particle-bound LH accounted for 42% of the total LH while cytoplasm contained 58% of the total immunoassayable LH in these extracts. 70% of particle-bound LH was present in the P2 fraction and most of the particle-associated LH was found in subfractions of P2 rich in synaptosomes but poor in myelin and mitochondria. While actual amounts of LH in the P3 and P4, the microsomal fractions, were relatively small, the concentration of LH, expressed as nanograms per milligram of protein was quite high. Both content and concentration of LH in the nuclear pellet, P1, were substantially lower. These findings provide further framework for speculation on the role of brain-based LH.
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