Long-term cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain (D) of Rous sarcoma virus
作者:
Lorraine Leblond-Larouche,
Réjean Morais,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1976)
卷期:
Volume 22,
issue 10
页码: 1474-1479
ISSN:0008-4166
年代: 1976
DOI:10.1139/m76-218
出版商: NRC Research Press
数据来源: NRC
摘要:
Attempts have been made to keep in vitro, for extended periods of time, cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain of Rous sarcoma virus, subgroup D. Roller cultures of transformed chick cells kept in serum-deficient medium can be maintained without subcultivation for up to 6 months. The confluent cultures continuously release viruses and viable tumor cells into the medium. The released cells can be plated and have characteristics of growth and morphology which are relatively stable with time until the culture degenerates. Cells released at later stages of the culture produced substantially more viruses than those released earlier, suggesting that cell selection or differentiation occurs during long-term cultivation in low serum concentration. Long-term cultures of untransformed chick embryo fibroblasts can also be maintained in the same way. The release of viable cells by these confluent cultures, however, is negligible.
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