Effects of piperonyl butoxide on dimethylnitrosamine metabolism and toxicity in Swiss mice
作者:
MarvinA. Friedman,
Virginia Sanders,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1976)
卷期:
Volume 2,
issue 1
页码: 67-75
ISSN:0098-4108
年代: 1976
DOI:10.1080/15287397609529418
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The effects of piperonyl butoxide (PB) on the metabolism and toxicity of dimethylnitrosamine (DMN) in Swiss mice were determined. PB, at doses of 10 and 20 mg/kg, inhibited DMN demethylase 45 min after treatment by 18 and 37%. These inhibitory effects were marked 0.5 hr after PB (640 mg/kg) treatment and reached maximal effects at 2 hr when there was 55% inhibition of enzyme activity. The inhibition by PB continued for 24 hr where enzyme activity was suppressed by 35%. At 48 hr after treatment there was stimulation of enzyme activity. Enzyme kinetic determinations showed no change in Kmbut Vmaxdecreased from 129 to 49 μmol CH2O/min‐g liver. PB (640 mg/kg) inhibited DMN (500 mg/kg; im) mutagenicity in the host‐mediated assay, decreasing the mutant frequency by 42%. Paradoxically, PB (640 mg/kg) had no effect on the alkylation of nucleic acids or proteins in mouse liver, kidney, lung, or spleen. In addition, pretreatment with PB (640 mg/kg) had no effect on the LD50of DMN.
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