AbstractVolatile substances emitted from the flowers of eightMagnoliataxa (M. sieboldiissp.japonica, M. praecocissimavar.praecocissimaand var.borealis, M. tomentosa, M. salicifolia, M. obovata, M. denudata, and M. grandiflora) and oneMicheliaspecies (M. compressa) (Magnoliaceae) were examined and identified using GC‐MS. Volatile substances of theseMagnoliaandMicheliaspecies consist primarily of monoterpenoids and sesquiterpenoids produced by the mevalonate pathway, acetogenins by the acetate‐malonate pathway, and phenyl‐propanoids by the shikimate pathway. TheseMagnoliaandMicheliaspecies all possessed various combinations of volatile monoterpenoids, acetogenins, and phenylpropanoids, except forMagnolia obovata, which emitted primarily sesquiterpenoids. Free amino acids in pollen of 12Magnoliaand oneLiriodendronspecies were also analyzed, and their value as food sources for pollinators evaluated.Pollinators visiting the flowers of fiveMagnoliaspecies were collected in their native sites and identified. Their behaviors and roles as pollinating agents were ass