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Oestrogen and Progesterone Receptors in Normal Human Endometrium: Comparison of Immunocytochemical Analysis on Frozen and Paraffin Sections with or without Enzymatic Pretreatment

 

作者: Hedy Yun Mei Fung,   Yuk Ling Wong,   Felix Wu Shun Wong,   Michael Scott Rogers,  

 

期刊: Gynecologic and Obstetric Investigation  (Karger Available online 1995)
卷期: Volume 39, issue 4  

页码: 262-265

 

ISSN:0378-7346

 

年代: 1995

 

DOI:10.1159/000292422

 

出版商: S. Karger AG

 

关键词: Oestrogen receptor;Progesterone receptor;Paraffin sections;Enzymatic pretreatment

 

数据来源: Karger

 

摘要:

Immunocytochemical study in paraffin sections of human endometrium showed that the receptor contents for both oestrogen and progesterone receptors were lower than in the frozen sections although the staining patterns were similar in these two section types. Pretreating the specimens with proteolytic enzymes like trypsin, DNase and pronase improved the oestrogen receptor staining but a better result with progesterone receptor staining was obtained when no enzymatic pretreatment was applied to the sections.

 

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