Oestrogen and Progesterone Receptors in Normal Human Endometrium: Comparison of Immunocytochemical Analysis on Frozen and Paraffin Sections with or without Enzymatic Pretreatment
作者:
Hedy Yun Mei Fung,
Yuk Ling Wong,
Felix Wu Shun Wong,
Michael Scott Rogers,
期刊:
Gynecologic and Obstetric Investigation
(Karger Available online 1995)
卷期:
Volume 39,
issue 4
页码: 262-265
ISSN:0378-7346
年代: 1995
DOI:10.1159/000292422
出版商: S. Karger AG
关键词: Oestrogen receptor;Progesterone receptor;Paraffin sections;Enzymatic pretreatment
数据来源: Karger
摘要:
Immunocytochemical study in paraffin sections of human endometrium showed that the receptor contents for both oestrogen and progesterone receptors were lower than in the frozen sections although the staining patterns were similar in these two section types. Pretreating the specimens with proteolytic enzymes like trypsin, DNase and pronase improved the oestrogen receptor staining but a better result with progesterone receptor staining was obtained when no enzymatic pretreatment was applied to the sections.
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