Immunodetection of a Tumor Associated Antigen (TAG-12): Comparison of Microwave Accelerated and Conventional Method
作者:
WernerMartin,
von WasielewskiReinhard,
GeorgiiAxel,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 2
页码: 79-81
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110554
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The microwave stimulated immunodetection of a tumor associated antigen (TAG-12) by monoclonal antibody 7A9 and an avidin-biotinylated alkaline phosphatase kit was compared with the conventional staining method. No difference in the staining pattern of antibody 7A9 was noticed in serial paraffin sections of 50 specimens including normal, benign and malignant breast tissues after microwave irradiated and conventional immunostaining. The results demonstrate that microwave stimulated immunostaining gives reliable results and can remarkably reduce the time of the staining procedure.
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