Insulin-like growth factor I stimulates proliferation, migration, and plasminogen activator release by human retinal pigment epithelial cells
作者:
GrantMaria B.,
GuayColleen,
MarshRobert,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 4
页码: 323-335
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689008999620
出版商: Taylor&Francis
数据来源: Taylor
摘要:
The migration of retinal pigment epithelial (RPE) cells from their normal anatomic position to a new position in the vitreous cavity is a critical feature of proliferative vitreous retinopathy. To determine if insulin-like growth factor I (IGF I), which is present in the vitreous fluid of diabetics, stimulates RPE cells, we examined the effects of IGF I on the proliferation, chemotaxis, and release of plasminoqen activator by these cells.At the concentrations of IGF I tested, significant proliferation of RPE cells is seen. Significant chemotaxis of the RPE cells also is seen at all the concentrations of IGF I tested. The mean number of migrating cells per high-powered field in control studies was 43±13 (x±SEM), and for IGF I at 2.5 ng and 50 ng/ml the mean numbers of migrating cells were 96±17 and 483±62, respectively (P<0.001 for each comparison). The IGF I response was noted to be dose-dependent. The chemotactic response noted at 50 ng/ml of IGF I was greater than the positive chemotactic control of 10% fetal calf serum. Addition ofαIR-3, an IGF I receptor antibody, eliminated the IGF I chemotactic response.The effect of IGF I on the secretion of plasminogen activators was assessed using an immunological assay for tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI). Media conditioned by RPE cells have measureable levels of PAI and t-PA antigen. IGF I supplementation resulted in an increase of t-PA secretion and PAI secretion over basal levels. These studies support a role for IGF I in modulating RPE cell function.
点击下载:
PDF (2599KB)
返 回