lnterleukin-2-lnduced Cytotoxicity of Chediak-Higashi Lymphocytes
作者:
Randall F. Holcombe,
期刊:
Acta Haematologica
(Karger Available online 1992)
卷期:
Volume 87,
issue 1-2
页码: 45-48
ISSN:0001-5792
年代: 1992
DOI:10.1159/000204712
出版商: S. Karger AG
关键词: Chediak-Higashi syndrome;Cytotoxicity;Interleukin 2;LAK cells;T cell;T cell receptor;gamma:delta
数据来源: Karger
摘要:
Peripheral blood mononuclear cells (PBMCs) from patients with Chediak-Higashi syndrome (CHS) exhibit minimal natural-killer-like cytotoxicity by standard 51Cr-release assays in vitro. However, a CHS-derived γδ-T-cell clone maintained in long-term culture with low-dose interleukin-2 (IL-2) does exhibit cytotoxic activity. PBMCs from 2 patients with CHS were stimulated with low-dose IL-2 in short-term culture and compared with a control for induction of cytotoxicity against a panel of target cell lines. The CHS-derived PBMCs exhibited IL-2-induced cytotoxicity, though the magnitude of induction was uniformly less than in controls. This was accompanied by no significant change in the proportion of PBMCs bearing the γδ-T-cell receptor antigens. The inducibility of cytotoxicity with low-dose IL-2 in vitro suggests a possible in vivo therapy for patients with
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