Resistance of mouse cytolytic cells to pore‐forming protein‐mediated cytolysis
作者:
Yo Shinkai,
Hitoshi Ishikawa,
Masakazu Hattori,
Ko Okuimira,
期刊:
European Journal of Immunology
(WILEY Available online 1988)
卷期:
Volume 18,
issue 1
页码: 29-33
ISSN:0014-2980
年代: 1988
DOI:10.1002/eji.1830180106
出版商: WILEY‐VCH Verlag GmbH
数据来源: WILEY
摘要:
AbstractPore‐forming protein (perforin, PFP) was isolated from a mouse large granular lymphocyte (LGL) [natural killer (NK‐like)] cell line. Purified PFP lysed a variety of mouse tumor cell lines and helper T lymphocyte cell lines. However, LGL and cytotoxic T lymphocyte cell lines were resistant to PFP‐mediated cell lysis. The presence of hemolytic activity in the granule was examined in these resistant cell lines. Four out of five of these resistant cell lines had hemolytically active granules. We determined whether NK cells freshly isolated from BALB/c nude mouse spleens were resistant to PFP‐mediated cytolysis. Nylon column‐passed spleen cells with an enriched content of NK cells exhibited more resistance than whole spleen cells. Moreover, when spleen cells were treated with PFP the remaining live cells showed enriched NK activity suggesting that normal peripheral cells with NK activity are resistant to PFP. These results indicate that cytolytic cells containing PFP have developed defense mechanisms to inhibit PEP‐mediated
点击下载:
PDF
(553KB)
返 回