Scanning tunneling microscopy of chloroplasts
作者:
B. Mainsbridge,
T. Thundat,
期刊:
Journal of Vacuum Science&Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena
(AIP Available online 1991)
卷期:
Volume 9,
issue 2
页码: 1259-1262
ISSN:1071-1023
年代: 1991
DOI:10.1116/1.585216
出版商: American Vacuum Society
关键词: CHLOROPLASTS;IMAGES;SCANNING TUNNELING MICROSCOPY;SOLUTIONS;GOLD;SUBSTRATES;ELECTROCHEMICAL COATING
数据来源: AIP
摘要:
Conventional preparation techniques as used in electron microscopy have been used to separate chloroplasts. While still in a buffer solution they have been electrochemically deposited on a gold substrate. Scanning tunneling microscope images of the grana and lamellae of spinach chloroplasts have been found to be consistent with evidence from electron microscopy of their external structure. Possible contact sites, where proteins are imported selectively into the membrane, have been observed, some supporting linear molecular chains approximately 10 nm long.
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