Survival and Axonal Elongation of Adult Rat Retinal Ganglion Cells
作者:
Solon Thanos,
Mathias Bähr,
Yves‐Alain Barde,
Jens Vanselow,
期刊:
European Journal of Neuroscience
(WILEY Available online 1989)
卷期:
Volume 1,
issue 1
页码: 19-26
ISSN:0953-816X
年代: 1989
DOI:10.1111/j.1460-9568.1989.tb00770.x
出版商: Blackwell Publishing Ltd
关键词: Axon regeneration;central neurons;fluorescent dyes;neurotrophic factors;immunohistochemistry
数据来源: WILEY
摘要:
AbstractA peripheral nerve exudate, collectedin situfrom the proximal end of a severed rat sciatic nerve, can induce substantial axonal elongation from ganglion cells when tested on explanted adult rat retinae. The responsive cells are identified on the basis of their Thy 1.1 immunostaining properties. Similar outgrowth can be obtained from explants when the culture medium is supplemented with brain‐derived neurotrophic factor (BDNF). In addition, both BDNF and the sciatic nerve exudate can prevent ganglion cell degeneration as shown by the retrograde transport of a fluorescent dye that had been applied to the superior colliculus prior to explantation. The results demonstrate that soluble components, released by lesioned peripheral nerves, can effect adult retinal ganglion cells in a way that is reminiscent of that which has been describedin vivousing sciatic nerve grafts after sectioning of the optic nerve. The molecular nature of these components is discusse
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