Monitoring of protein product formation during fermentation with fast protein liquid chromatography
作者:
Jan‐Gunnar Gustafsson,
Ann‐Kristin Frej,
Per Hedman,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1986)
卷期:
Volume 28,
issue 1
页码: 16-20
ISSN:0006-3592
年代: 1986
DOI:10.1002/bit.260280104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA method is described using fast protein liquid chromatography (FPLC) for the monitoring of protein formation during fermentation. The procedure consists of centrifugation to recover the cells, sonication of the cells, centrifugation to remove cell debris, and analysis of supernatant on a column of Mono Q (a strong anion exchanger). Analysis of peak areas provides quantitative determination of product concentration. Maintenance and life of the Mono Q column is discussed. We find that FPLC is a convenient method for measuring products in cell homogenates because it gives rapid, highly resolved separations.
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