The Enzymology and Intracellular Organization of Peptide Precursor Processing: The Secretory Vesicle Hypothesis
作者:
Harold Gainer,
James T. Russell,
Y. Peng Loh,
期刊:
Neuroendocrinology
(Karger Available online 1985)
卷期:
Volume 40,
issue 2
页码: 171-184
ISSN:0028-3835
年代: 1985
DOI:10.1159/000124070
出版商: S. Karger AG
关键词: Prohormone-processing enzymes;Secretory vesicle;Aminopeptidase;Carboxypeptidase B;α-Amidation;ATPase;Cytochrome b561;Prohormones
数据来源: Karger
摘要:
The ‘secretory vesicle hypothesis of precursor processing’ states that the initial endopeptidase cleavages which excise the nascent, biologically active peptides from their protein precursors occur primarily in secretory vesicles (or granules). Hence, all the processing steps subsequent to these cleavages must also occur within these organelles. Two types of evidence are presented in support of this view: (1) cell biological studies which implicate the secretory vesicle as the site of precursor conversion to peptides, and (2) enzymological studies which locate and characterize putative processing enzymes in secretory vesicles. The processing enzymes reviewed include the ‘prohormone-converting enzymes’ which cleave at pairs of basic amino acids, other endopeptidases, carboxypeptidase-B-like enzymes and aminopeptidase, and N-acetylation and α-amidation enzymes. The properties of these enzymes in relation to the nature of the processing micro-environment in the secretory vesicles is d
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