Active Sites of Dermatan Sulfate for Heparin Cofactor II. Isolation of a Nonasaccharide Fragment Containing Four Disaccharide Sequences [α-l-Iduronic Acid 2-O-Sulfate (1,3)-β-d-N-Acetylgalactosamine 4-Sulfate]
作者:
G. Mascellani,
L. Liverani,
A. Prete,
G.L. Bergonzini,
P. Bianchini,
L. Silvestro,
G. Torri,
A. Bisio,
M. Guerrini,
B. Casu,
期刊:
Journal of Carbohydrate Chemistry
(Taylor Available online 1995)
卷期:
Volume 14,
issue 8
页码: 1165-1177
ISSN:0732-8303
年代: 1995
DOI:10.1080/07328309508005402
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The active site of dermatan sulfate (DS) for heparin cofactor II (HCII) was isolated in a fragment obtained by periodate oxidation, borohydride reduction, mild acid hydrolysis, and SE- and SAX-chromatography of beef mucosal and pig skin DS preparations. Characterization by mass spectrometry, one- and two-dimensional NMR spectroscopy, and HPLC analysis of disaccharides, obtained by exhaustive digestion with chondroitinase-ABC, indicates that the fragment has the prevalent structure1, GalNAc-4SO3-[IdoA-2SO3-GalNAc-4SO3]4-R, where R is CH(CH2OH)CH(COO−)-OH.1, is the largest DS fragment thus far isolated containing IdoA2SO3as the only uronic acid. Its lower activity (30%) with respect to the parent polymeric DS is explainable by Tollefsen model, requiring longer polyanionic chains for formation of ternary complex with thrombin.
点击下载:
PDF (550KB)
返 回