Summary—With specific antibodies directed against non‐actin micrifilaments (NAMFs), it was possible to determine the spatial distribution of these cytoskeletal elements within the cell cortex of the ciliateClimacostomum virensby immunofluorescence. In the somatic areas, the antibodies allowed to vizualize a more or less continuous layer spanning the whole cell surface with a higher amount of filaments just beneath the ciliary rows. In the buccal region the NAMF system forms bundles running parallel to the ciliary clusters termed the membranelles. The same procedure was used on cells treated with 1 M urea, an agent which induces the release of the oral apparatus followed by a complete regeneration of this part of the cell body. Such an approach completed by an electron microscopy study allowed us to describe the dynamic of NAMFs in the region where new ciliary membranelles will organize from a few somatic kinetics. We observed that in this region, the NAMF system undergoes a desorganization‐reorganization cycle during oral apparatus regeneration. This cycle may be related to the destabilisation of the cortex which allows kinetosome proliferation and to its restabilisation which corresponds to the definitive positioning of the new kinetosomes. Consequently, regeneration ofC virens'soral apparatus seems to be a good model for studying relations between cortical NAMF cytoskeleton, positioning of kinetosomes, and cortical stabilis