Petromyces albertensisproduced xylitol andD-xylulose when cultivated on a medium containingD-xylose. These fermentative products were identified by high-performance liquid chromatography. A large amount of xylitol was obtained from aD-xylose medium containing ammonium acetate and yeast extract at an initial pH of 7.0. Maximum production of xylitol and of the enzymes concerned with its production was observed after 10 days of cultivation. AD-xylose (100 g/L) medium supplemented with 1% (v/v) methanol gave the highest yields of xylitol (39.8 g/L) andD-xylulose (2.8 g/L).Key words:Petromyces albertensis,D-xylulose, xylitol.