The preferential distribution of the relatively nonpolar pesticide chlordecone (CD) to liver rather than to fat tissues in humans suggests that it may be transported in plasma differently from other organochlorine pesticides. The plasma binding of [14C]CD was investigatedin vitroin human, rat, and pig plasma andin vivoin rat plasma. Protein and lipoprotein fractions were separated by serial ultracentrifugation. Heparin‐manganese precipitation and agorose gel electrophoresis were also carried out to determine whether separation techniques altered CD binding to plasma components.