Determination of Identity in Paraffin-Embedded Tissues and Slides
作者:
I. Medintz,
L. Chiriboga,
L. McCurdy,
R.K. Garg,
L. Kobilinsky,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 15
页码: 2625-2633
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508007414
出版商: Taylor & Francis Group
关键词: DNA;PCR;Paraffin;Slide;Donor;Identity Forensic
数据来源: Taylor
摘要:
A procedure is presented for the determination of donor identity or commonality of origin among paraffinembedded tissues and slides. Samples are de-paraffinized with xylene and ethanol and subjected to ChelexRextraction. The Polymarker forensic DNA typing kit is utilized for identity testing. Following multiplex amplification, PCR product is verified on an agarose gel and then the samples are geiotyped using reverse dot-blot hybridization. When this procedure was applied to paraffin-embedded samples involved in a legal action, all samples were successfully genotyped. The advantages of using this procedure are discussed.
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