Differential morphometric values induced in Golgi apparatus of higher plant cells by aldehyde and permanganate fixation
作者:
José A. Gonzalez‐Reyes,
Francisco Gracia‐Navarro,
Gregorio Garcia‐Herdugo,
Antonio Hidalgo,
Plácido Navas,
期刊:
Journal of Electron Microscopy Technique
(WILEY Available online 1989)
卷期:
Volume 11,
issue 1
页码: 1-8
ISSN:0741-0581
年代: 1989
DOI:10.1002/jemt.1060110102
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Electron microscopy;Fixation methods;Golgi apparatus morphometry;Onion roots
数据来源: WILEY
摘要:
AbstractIn order to determine the best conditions to carry out quantitative ultrastructural studies in plant specimens, five different fixation techniques, including some of the most reported electron microscopy fixatives (glutaraldehyde‐paraformaldehyde, osmium tetroxide, potassium permanganate), were assayed in onion root meristems to check their ability to induce morphometric changes in Golgi apparatus ultrastructure. Although the parameters evaluated showed in all cases the same tendency, values obtained after permanganate fixation were always higher than those found after aldehyde techniques (especially aldehyde‐osmium). Aldehyde followed by osmium fixation appears as the most indicated fixation method when accurate quantitative ultrastructural studies are to be develo
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