Intracerebroventricular Injection of Tumor Necrosis Factor-αInduces Thermal Hyperalgesia in Rats
作者:
Takakazu Oka,
Yoshiyuki Wakugawa,
Masako Hosoi,
Kae Oka,
Tetsuro Hori,
期刊:
Neuroimmunomodulation
(Karger Available online 1996)
卷期:
Volume 3,
issue 2-3
页码: 135-140
ISSN:1021-7401
年代: 1996
DOI:10.1159/000097238
出版商: S. Karger AG
关键词: Tumor necrosis factor-α;Hyperalgesia;Prostaglandin;Interleukin-1;Pain;Central nervous system;Rat
数据来源: Karger
摘要:
To investigate the role of tumor necrosis factor-α(TNF-α) in the brain in nociception, we injected recombinant human TNF-α(rhTNF-α; 1 pg–10 ng/rat) into the lateral cerebroventricle (LCV) in rats and observed the changes in paw withdrawal latency to radiant heat by using the plantar test for 90 min after injection. LCV injections of TNF-αat doses of 10 pg, 100 pg and 1 ng reduced paw withdrawal latency, showing a maximal response at a dose of 10 pg which peaked 60 min after injection. TNF-αat doses of 1 pg and 10 ng had no effect on nociception during the test period. The TNF-α(10 pg)-induced reduction in paw withdrawal latency was blocked by simultaneous injection of diclofenac (1 ng), a cyclooxygenase inhibitor, or interleukin-1 receptor antagonist (IL-1ra, 10 ng). LCV injection of neither diclofenac (1 ng) nor IL-1ra (10 ng) had any effect on nociception by itself. The results suggest that TNF-αin the brain induces thermal hyperalgesia and that the brain TNF-α-induced hyperalgesia is mediated by the central action of interleukin-1 and activation of the cyclooxygenase pathway of the ar
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