Optimization of culture conditions for the formation of sperm cells in pollen tubes of tradescantia
作者:
Gloria J. Lafleur,
Amy E. Gross,
Joseph P. Mascarenhas,
期刊:
Gamete Research
(WILEY Available online 1981)
卷期:
Volume 4,
issue 1
页码: 35-40
ISSN:0148-7280
年代: 1981
DOI:10.1002/mrd.1120040106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: sperm;pollen tube;culture media
数据来源: WILEY
摘要:
AbstractA culture medium and culture conditions are described that enable generative cell division and sperm formation to occur in a large proportion (greater than 70%) of the pollen tubes of Tradescantia paludosa within six to eight hours of culture of pollen. The nature of the nitrogen source, speed of shaking, and ratio of pollen to medium are important parameters in determining the extent of sperm formation. Addition of the plant hormones indole acetic acid, gibberellic acid, and kinetin to the growth medium does not influence generative cell division.
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