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A Model On-line Flow Injection Fluorescence Immunoassay Using a Protein a Immunoreactor and Lucifer Yellow

 

作者: DerekA. Palmer,   Ren Xuezhen,   Pilar Fernandez-Hernando,   JamesN. Miller,  

 

期刊: Analytical Letters  （Taylor Available online 1993）
卷期: Volume 26, issue 12  

页码: 2543-2553

 

ISSN:0003-2719

 

年代: 1993

 

DOI:10.1080/00032719308017972

 

出版商: Taylor & Francis Group

 

关键词: Transferrin;heterogeneous fluorescence immunoassay;flow injection analysis;protein A;lucifer yellow.

 

数据来源: Taylor

 

摘要:

A heterogeneous on-line fluorescence immunoassay has been developed for a model analyte (transferrin) using a flow injection analysis system containing a controlled pore glass protein A immunoreactor. Lucifer yellow VS (LYVS) a 4-aminonaphthalimide with a large stokes shift and pH independence was the fluorophore. For each assay the antibody-protein A reaction takes place at near neutral pH, and the complexes are eluted at acid pH. Transferrin levels in human serum has been determined by this method, and good within assay variations have been achieved.

 

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