DETERMINATION OF HYOSCINE N-BUTYL-BROMIDE, LIDOCAINE HYDROCHLORIDE, AND PARACETAMOL IN INJECTION FORMS USING SOLID-PHASE EXTRACTION, HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, AND UV-VIS SPECTROPHOTOMETRY
作者:
M. Parissi-Poulou,
I. Panderi,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 7
页码: 1055-1068
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101717
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A solid phase extraction procedure using strong cation exchange (SCX, benzenesulfonic acid) cartridges followed by a reversed-phase HPLC assay was applied to the analysis of hyoscine n-butylbromide and lidocaine hydrochloride in injection forms. The chromatographic separation was performed on a BDS C-18 column. The mobile phase consisted of a mixture of acetonitrile: ammonium acetate 0.2M, (30:70, v/v) pumped at a flow rate 1.2 mL/min. The UV detector was operated at 254 nm. A UV-Vis spectrophotometric method was also developed for the determination of paracetamol in the injection forms. The method consists of subsequent dilution of the injection forms and measure of the absorbance value at 242.7 nm. Relative standard deviation was less than 0.95% for HPLC and less than 0.78% for the spectrophotometric method. Detection limits were 1.05, 0.96 and 0.67 μg/mL for hyoscine n-butylbromide, lidocaine hydrochloride and paracetamol, respectively.
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