首页   按字顺浏览 期刊浏览 卷期浏览 Combinatorial Libraries
Combinatorial Libraries

 

作者: PerssonMats A. A.,  

 

期刊: International Reviews of Immunology  (Taylor Available online 1993)
卷期: Volume 10, issue 2-3  

页码: 153-163

 

ISSN:0883-0185

 

年代: 1993

 

DOI:10.3109/08830189309061692

 

出版商: Taylor&Francis

 

关键词: antibodies;phage iamboa;filamentous phage;phage display;cDNA;repertoire cloning

 

数据来源: Taylor

 

摘要:

Combinatorial antibody libraries, in which PCR amplified immunoglobulin light and heavy chain DNA are randomly recombined irrespective of their pairing in vivo into a vector and subsequently expressed in E. coli, have quickly become a very productive tool to generate monoclonal antibodies from various species. It has been drastically improved by utilizing phage display technologies in the selection process of specific antibodies. A brief summary of current techniques, critical published experiments showing the versatility of these systems with emphasis on human antibodies and discussions on chain preference, affinity maturation and the advent of semisynthetic and non-immune libraries will be presented.

 

点击下载:  PDF (821KB)



返 回