Combinatorial Libraries
作者:
PerssonMats A. A.,
期刊:
International Reviews of Immunology
(Taylor Available online 1993)
卷期:
Volume 10,
issue 2-3
页码: 153-163
ISSN:0883-0185
年代: 1993
DOI:10.3109/08830189309061692
出版商: Taylor&Francis
关键词: antibodies;phage iamboa;filamentous phage;phage display;cDNA;repertoire cloning
数据来源: Taylor
摘要:
Combinatorial antibody libraries, in which PCR amplified immunoglobulin light and heavy chain DNA are randomly recombined irrespective of their pairing in vivo into a vector and subsequently expressed in E. coli, have quickly become a very productive tool to generate monoclonal antibodies from various species. It has been drastically improved by utilizing phage display technologies in the selection process of specific antibodies. A brief summary of current techniques, critical published experiments showing the versatility of these systems with emphasis on human antibodies and discussions on chain preference, affinity maturation and the advent of semisynthetic and non-immune libraries will be presented.
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