Flow-Through Immunoassay System for Determination of Staphylococcal Protein A
作者:
Ihab Abdel-Hamid,
Andrey L. Ghindilis,
Plamen Atanasov,
Ebtisam Wilkins,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 6
页码: 1081-1094
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542879
出版商: Taylor & Francis Group
关键词: amperometric immunosensor;protein A;Staphylococcus aureus
数据来源: Taylor
摘要:
An amperometric immunosensor for fast immunoassay of protein A has been developed. The disposable immunoelectrode consists of highly dispersed carbon modified by immobilized anti-protein A antibodies. The flow-through technique permits high automation of the entire measuring procedure. The detection is based on peroxidase-labeled anti-IgG antibodies captured by the immunoelectrode as a result of immunointeraction. The immunoassay employing a 'sandwich' scheme for the determination of different proteins with a detection limit in the nanomolar range is demonstrated. The immunosensor allows fast determination of protein A with an overall analysis time as low as 22 min. It can be further developed forStaphylococcus aureusassay based on the protein A expressed by this microorganism. The immunoassay system can be arranged as a portable device for fast analyses. The environmental application of the device is associated with on-site monitoring of bacterial contamination under field conditions.
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