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Comprehensive Evaluation of Acute Immunological Changes Induced by Cuprophane and Polysulfone Membranes in a Patient on Chronic Hemodialysis

 

作者: Roberto Patarca,   Guido Perez,   Anthony Gonzalez,   Rolando 0. Garcia-Morales,   Roberto Gamble,   Nancy Klimas,   Mary Ann Fletcher,  

 

期刊: American Journal of Nephrology  （Karger Available online 1992）
卷期: Volume 12, issue 4  

页码: 274-278

 

ISSN:0250-8095

 

年代: 1992

 

DOI:10.1159/000168459

 

出版商: S. Karger AG

 

关键词: Cuprophane;Polysulfone;Cytokines;mRNA;Phytohemagglutinin;Lymphoid cell phenotypes;Polymerase chain reaction

 

数据来源: Karger

 

摘要:

We have investigated the acute changes in cytokine gene expression patterns and immune status in vivo and ex vivo in a patient on chronic hemodialysis with cuprophane membranes before and after two hemodialysis sessions with either cuprophane or polysulfone membranes. Results show that both dialyzer membranes have differential acute effects on the immune system and that the concomitant use of reverse-transcriptase-coupled polymerase chain reaction, solid-phase and radioimmunoassays, and flow-cytometric analysis may be useful in the longitudinal assessment of patients on chronic hemodialysis.

 

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