MULTIDIMENSIONAL MULTIMODAL INSTRUMENTAL SEPARATION OF COMPLEX MIXTURES
作者:
HaleemJ. Issaq,
KingC. Chan,
GeorgeM. Janini,
GaryM. Muschik,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 1
页码: 145-154
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101441
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Presented here is a simple approach to the application of HPLC/CE for the separation of complex mixtures. A two-dimensional HPLC/CE instrumental setup was assembled from commercially available equipment. Fractions of the effluent from the HPLC system are collected into microtiter plates with the aid of a microfraction collector which allows the collection of samples by time, drops, or external signal (peaks). The fractions are then dried under vacuum at room temperature in a special unit, reconstituted, and analyzed by CZE. Any size or type of HPLC or CE column can be used with no limitation on the amount of sample injected into the HPLC. Any CE detection procedure, such as LIF, MS, UV, or other, can be used. This setup is practical, simple, robust, and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of a mixture of two protein digests, cytochrome C and myoglobin.
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