Expression of Cell Adhesion Molecules in Corneal Graft Failure
作者:
Scott Whitcup,
Robert Nussenblatt,
Francis Price,
Chi-Chao Chan,
期刊:
Cornea
(OVID Available online 1993)
卷期:
Volume 12,
issue 6
页码: 475-480
ISSN:0277-3740
年代: 1993
出版商: OVID
关键词: Cell adhesion molecules;Graft failure
数据来源: OVID
摘要:
Corneal graft failure is frequently mediated by uncontrolled inflammatory disease. We studied the expression of cell adhesion molecules in seven penetrating keratoplasty specimens with graft failure and in a normal eye bank cornea using immunohistochemical staining and monoclonal antibodies against intercellular adhesion molecule- 1 (ICAM-1, CD54), lymphocyte functionassociated antigen-1 (LFA-1, CD1 la/CD 18), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and major histocompatibility complex (MHC) class II antigen (HLA-DR). ICAM-1 and HLA-DR were expressed on keratocytes and the corneal endothelium in six of the seven specimens. ICAM-1 expression was strongest in the corneas with the most severe inflammation (corneal allograft rejection and severe intraocular inflammation). LFA-1 is a counter-receptor for ICAM-1, and infiltration with leukocytes expressing either the a or (J chain of LFA-1 was found in areas of ICAM-1 expression in four of the seven corneas. In contrast, E-selectin was expressed in the stroma in only two specimens, and VCAM-1 in one specimen. Expression of cell adhesion molecules or MHC class II antigen were not detected in the normal eye bank cornea. These data suggest that ICAM-1 expression may play an important role in the development of corneal graft failure. Furthermore, monoclonal antibodies to block ICAM-1 or its ligands may inhibit the development of corneal inflammation.
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