Selective Monitoring of Stern-Volmer Quenching of Chiral Molecules Via Fluorescence Detected Circular Dichroism
作者:
MarkP. Thomas,
Gabor Patonay,
IsiahM. Warner,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 5
页码: 717-730
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708062923
出版商: Taylor & Francis Group
关键词: Fluorescence Detected Circular Dichroism;Fluorescence Quenching;Circular Dichroism
数据来源: Taylor
摘要:
The use of fluorescence detected circular dichroism (FDCD) for monitoring the quenching of chiral molecules is described. This technique combines the specificity for chiral centers of circular dichroism (CD), the sensitivity and selectivity of the fluorescence measurement with the additional chemical information provided by fluorescence quenching. Spectra are acquired using a multidimensional FDCD spectrometer which obtains a FDCD matrix of spectral data as a function of multiple excitation wavelengths (CD information) and multiple fluorescence emission wavelengths. This combination of FDCD and fluorescence quenching produces a very selective monitor for chiral fluorophores.
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