Measurement of Light‐Dependent Changes of the Stromal pH in Wheat Leaf Protoplasts
作者:
D. Heineke,
H. W. Heldt,
期刊:
Botanica Acta
(WILEY Available online 1988)
卷期:
Volume 101,
issue 1
页码: 45-47
ISSN:0932-8629
年代: 1988
DOI:10.1111/j.1438-8677.1988.tb00010.x
出版商: Blackwell Publishing Ltd
关键词: Chloroplasts;wheat leaf protoplasts;pH stroma
数据来源: WILEY
摘要:
AbstractThe stromal pH of chloroplasts contained in wheat leaf protoplasts was determined by incubating the protoplast suspension with radioactively labelled DMO, followed by rapid subcellular fractionation and measurement of the radioactivity in the separated chloroplast fraction. The stromal pH was found to be about 8.0 upon illumination and 7.6 in darkness. These results demonstrate that light‐dependent stromal pH changes, which have been found earlier in isolated chloroplasts, also occur in a plant cel
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