Uptake and distribution of the nutrients iron and manganese by soybean [Glycine max(L.) Merr.] from a sewage sludge amended Enon sandy loam were investigated in the greenhouse. The sludge rates were 0, 40, 80, and 120 Mg ha−1dry basis. Soybeans of Bragg and Ransom cultivars were grown in pots to maturity. There was a significant increase in bean yield by plants grown in sludge amended soil compared to control. At maturity, the seeds, podwalls, and stems were sampled for the nutrient determination. As the rate of sludge applied Fe increased, there was a decrease in uptake of Fe by seed of Bragg, compared to control. The uptake of Fe in Bragg stem and podwall were unaffected by the sludge treatments. With an increased rate of sludge applied Fe, there was a decrease in Fe accumulation by stem of Ransom, whereas the podwall showed an increase. The uptake of Fe in Ransom seed was not affected by sludge treatment. Uptake of Mn decreased with increased rate of sludge applied Mn, by both the cultivars. Bragg seed accumulated more Fe than Ransom seed. The distribution of Fe was greater to seed compared to the other tissues for both the cultiv ars. Bragg stem and podwall accumulated higher Mn than Ransom. The distribution of Mn was greater to podwall compared to the other tissues for both the cultivars. There was no significant change in soil pH due to sludge amendment in this study.